FRANCESCO LIMONE

POSTDOCTORAL FELLOW

francesco.limone@nyulangone.org | @sciencelemon92

Born and raised in Italy, Francesco obtained his Bachelors in Molecular Cell Biology from University of York, UK, in 2015 and pursued his Masters in Stem Cell and Developmental Biology at Pierre et Marie Curie University – Sorbonnes Universités with École Normale Superieure, Paris, France in 2016.

Francesco continued is graduate work in Kevin Eggan’s lab in the Department of Stem Cell and Regenerative Biology at Harvard University. His work focused on single-cell RNA sequencing studies of in vitro human iPSC-derived systems and human primary samples to understand glial contributions to mechanisms of neurodegeneration in Amyotrophic Lateral Sclerosis (ALS) in collaboration with Beth Stevens’ lab. He also worked on the development of new differentiation schemes from hiPSCs and multiplexed single-cell RNA-sequencing analysis tools for the study of spinal cord motor neuron biology and ALS with the McCarroll and Nehme labs.

As a postdoctoral fellow in the Liddelow lab, Francesco will be studying the role of astrocytes in supporting neurons undergoing stress with the hope to widen our understanding of the role of glial-neuronal communication and manipulate glial cells in degenerative contexts to support neuronal survival.

KEY PUBLICATIONS

(* = equal contribution)

  1. Limone F*, Mordes D*, Couto A, Burberry A, Pietiläinen O, Joseph B, Ghosh S, Meyer D, Goldman M, Bortolin L, Kadiu I, McCarroll S, Eggan K. Single-nucleus sequencing reveals enriched expression of genetic risk factors in extratelencephalic neurons sensitive to degeneration in ALS. bioRxiv 2021.07.12.452054.

  2. Limone F, Mitchell JM, Guerra San Juan I, Ghosh S, Smith JLM, Raghunathan K, Couto A, Meyer D, Mello C, Nemesh J, Smith BM, McCarroll S, Pietiläinen O, Nehme R, Eggan K (2023) Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42(1):111896. PMID: 36596304.

  3. Klim J*, Williams L*, Limone F, Guerra San Juan I, Davis-Dusenbery B, Mordes D, Burberry A, Steinbaugh MJ, Gamage K, Kirchner R, Moccia R, Cassel S, Chen K, Wainger B, Woolf C, Eggan K (2019) ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair. Nat Neurosci 22(2):167-179. PMID: 30643292.